Journal: BMC Microbiology
Article Title: Characterising the metabolic functionality of the preterm neonatal gut microbiome prior to the onset of necrotising enterocolitis: a pilot study
doi: 10.1186/s12866-024-03701-x
Figure Lengend Snippet: Method for assessing carbon source utilisation by neonatal gut microbiome communities. (i) Neonatal stool was suspended in anaerobic Biolog Inoculation Fluid. (ii) Stool suspension was added to each well of a Biolog AN Microplate containing a different carbon source (iii) The plate was incubated in a plate reader, within an anaerobic chamber, at 37°C for 24 h. Redox dye in the plate changes colour, giving a measure of bacterial metabolic activity and thus carbon source utilisation. (iv) After incubation, dye change in each well gives a plate-wide pattern of carbon source utilisation for the microbial community
Article Snippet: Fig. 1 Method for assessing carbon source utilisation by neonatal gut microbiome communities. (i) Neonatal stool was suspended in anaerobic Biolog Inoculation Fluid. (ii) Stool suspension was added to each well of a Biolog AN Microplate containing a different carbon source (iii) The plate was incubated in a plate reader, within an anaerobic chamber, at 37°C for 24 h. Redox dye in the plate changes colour, giving a measure of bacterial metabolic activity and thus carbon source utilisation. (iv) After incubation, dye change in each well gives a plate-wide pattern of carbon source utilisation for the microbial community Here we present a pilot study in which for the first time we directly investigated the metabolic functionality of the NEC gut microbiome.
Techniques: Suspension, Incubation, Activity Assay