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Biolog Inc biolog inoculating fluid
Biolog Inoculating Fluid, supplied by Biolog Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biolog+inoculating+fluid/biolog+fluid+inoculating/pm42250346-45-7-7
Average 86 stars, based on 1 article reviews
biolog inoculating fluid - by Bioz Stars, 2026-09
86/100 stars

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Bacteria:

Article Title: The Lumen of Human Intestinal Organoids Poses Greater Stress to Bacteria Compared to the Germ-Free Mouse Intestine: Escherichia coli Deficient in RpoS as a Colonization Probe
Article Snippet: The metabolic properties of wild-type and ΔrpoS mutant ECOR2 were assessed with Biolog PM1 plates according to the manufacturer’s instructions. .. Bacteria grown overnight on LB agar were resuspended in Biolog inoculating fluid to a final OD600 of 1.0. .. Each well of the Biolog plate was inoculated with 100 l of the cell suspension and incubated in a BioTek kinetic plate reader (BioTek Instruments, Winooski, VT) for 24 h. The OD595 was used to measure the reduction of tetrazolium violet dye every 15 min.

Article Title: The Lumen of Human Intestinal Organoids Poses Greater Stress to Bacteria Compared to the Germ-Free Mouse Intestine: Escherichia coli Deficient in RpoS as a Colonization Probe
Article Snippet: The metabolic properties of wild-type and Δ rpoS mutant ECOR2 were assessed with Biolog PM1 plates according to the manufacturer’s instructions. .. Bacteria grown overnight on LB agar were resuspended in Biolog inoculating fluid to a final OD 600 of 1.0. .. Each well of the Biolog plate was inoculated with 100 μl of the cell suspension and incubated in a BioTek kinetic plate reader (BioTek Instruments, Winooski, VT) for 24 h. The OD 595 was used to measure the reduction of tetrazolium violet dye every 15 min.

Suspension:

Article Title: Master regulator NtrC controls the utilization of alternative nitrogen sources in Pseudomonas stutzeri A1501
Article Snippet: .. A total of 100 mL of this cell suspension inoculated into the Biolog inoculating fluid was transferred into each well and incubated at 30 °C for 24 h. The colour intensity was measured every 15 min using the OmniLog reader. ..

Article Title: Salinispora pacifica sp. nov., an actinomycete from marine sediments.
Article Snippet: Assimilation of a broad range of substrates was determined using Biolog GP2 plates. .. To this end, a mixture of black spores and mycelial fragments of each of the strains was obtained by centrifugation of modified Bennett’s broths (Jones 1949) prepared using 75 % seawater, following incubation at 28 C for 14 days; the resultant biomass was washed in sterile distilled water, centrifuged and then suspended in Biolog inoculating fluid to give a standard suspension equivalent to McFarland scale 5. ..

Article Title: Interspecies Transfer and Regulation of Pseudomonas stutzeri A1501 Nitrogen Fixation Island in Escherichia coli.
Article Snippet: 1Key Laboratory of Bio-resources and Eco-environment Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan 610064, P.R.. China 2Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.. China 3CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, P.R.

Incubation:

Article Title: Master regulator NtrC controls the utilization of alternative nitrogen sources in Pseudomonas stutzeri A1501
Article Snippet: .. A total of 100 mL of this cell suspension inoculated into the Biolog inoculating fluid was transferred into each well and incubated at 30 °C for 24 h. The colour intensity was measured every 15 min using the OmniLog reader. ..

Article Title: Salinispora pacifica sp. nov., an actinomycete from marine sediments.
Article Snippet: Assimilation of a broad range of substrates was determined using Biolog GP2 plates. .. To this end, a mixture of black spores and mycelial fragments of each of the strains was obtained by centrifugation of modified Bennett’s broths (Jones 1949) prepared using 75 % seawater, following incubation at 28 C for 14 days; the resultant biomass was washed in sterile distilled water, centrifuged and then suspended in Biolog inoculating fluid to give a standard suspension equivalent to McFarland scale 5. ..

Centrifugation:

Article Title: Salinispora pacifica sp. nov., an actinomycete from marine sediments.
Article Snippet: Assimilation of a broad range of substrates was determined using Biolog GP2 plates. .. To this end, a mixture of black spores and mycelial fragments of each of the strains was obtained by centrifugation of modified Bennett’s broths (Jones 1949) prepared using 75 % seawater, following incubation at 28 C for 14 days; the resultant biomass was washed in sterile distilled water, centrifuged and then suspended in Biolog inoculating fluid to give a standard suspension equivalent to McFarland scale 5. ..

Modification:

Article Title: Salinispora pacifica sp. nov., an actinomycete from marine sediments.
Article Snippet: Assimilation of a broad range of substrates was determined using Biolog GP2 plates. .. To this end, a mixture of black spores and mycelial fragments of each of the strains was obtained by centrifugation of modified Bennett’s broths (Jones 1949) prepared using 75 % seawater, following incubation at 28 C for 14 days; the resultant biomass was washed in sterile distilled water, centrifuged and then suspended in Biolog inoculating fluid to give a standard suspension equivalent to McFarland scale 5. ..

Sterility:

Article Title: Salinispora pacifica sp. nov., an actinomycete from marine sediments.
Article Snippet: Assimilation of a broad range of substrates was determined using Biolog GP2 plates. .. To this end, a mixture of black spores and mycelial fragments of each of the strains was obtained by centrifugation of modified Bennett’s broths (Jones 1949) prepared using 75 % seawater, following incubation at 28 C for 14 days; the resultant biomass was washed in sterile distilled water, centrifuged and then suspended in Biolog inoculating fluid to give a standard suspension equivalent to McFarland scale 5. ..



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Method for assessing carbon source utilisation by neonatal gut microbiome communities. (i) Neonatal stool was suspended in anaerobic Biolog Inoculation Fluid. (ii) Stool suspension was added to each well of a Biolog AN Microplate containing a different carbon source (iii) The plate was incubated in a plate reader, within an anaerobic chamber, at 37°C for 24 h. Redox dye in the plate changes colour, giving a measure of bacterial metabolic activity and thus carbon source utilisation. (iv) After incubation, dye change in each well gives a plate-wide pattern of carbon source utilisation for the microbial community

Journal: BMC Microbiology

Article Title: Characterising the metabolic functionality of the preterm neonatal gut microbiome prior to the onset of necrotising enterocolitis: a pilot study

doi: 10.1186/s12866-024-03701-x

Figure Lengend Snippet: Method for assessing carbon source utilisation by neonatal gut microbiome communities. (i) Neonatal stool was suspended in anaerobic Biolog Inoculation Fluid. (ii) Stool suspension was added to each well of a Biolog AN Microplate containing a different carbon source (iii) The plate was incubated in a plate reader, within an anaerobic chamber, at 37°C for 24 h. Redox dye in the plate changes colour, giving a measure of bacterial metabolic activity and thus carbon source utilisation. (iv) After incubation, dye change in each well gives a plate-wide pattern of carbon source utilisation for the microbial community

Article Snippet: Fig. 1 Method for assessing carbon source utilisation by neonatal gut microbiome communities. (i) Neonatal stool was suspended in anaerobic Biolog Inoculation Fluid. (ii) Stool suspension was added to each well of a Biolog AN Microplate containing a different carbon source (iii) The plate was incubated in a plate reader, within an anaerobic chamber, at 37°C for 24 h. Redox dye in the plate changes colour, giving a measure of bacterial metabolic activity and thus carbon source utilisation. (iv) After incubation, dye change in each well gives a plate-wide pattern of carbon source utilisation for the microbial community Here we present a pilot study in which for the first time we directly investigated the metabolic functionality of the NEC gut microbiome.

Techniques: Suspension, Incubation, Activity Assay